Genetics and pathophysiology of neuromuscular disorders linked to the extracellular matrix and to the nucleus
Genetics and pathophysiology of neuromuscular disorders linked to the extracellular matrix and to the nucleus
Our research interests focus on two groups of neuromuscular disorders (NMD): myopathies due to abnormalities of the myomatrix and of the nucleus. The long-term objective of our work is to propose relevant therapeutic options based on our knowledge of the genetic basis and of the underlying pathomechanisms at play in these rare diseases.
Research Project: Genetics and pathophysiology of neuromuscular disorders linked to the extracellular matrix and to the nucleus
Our team focuses on 2 groups of neuromuscular disorders: myopathies due to the defective myomatrix (collagen VI and other components of the extracellular matrix) and to defects of the myonucleus (Emery-Dreifuss muscular dystrophy and other striated muscle laminopathies due to mutations in the laminA/C gene or genes encoding components of nuclear membrane). These myopathies share some clinical features, notably prominent contractures, and constitute differential diagnosis[in1] .
These disorders are highly heterogeneous, clinically and genetically, and to date no treatment is available. Our previous work led us to identify the involvement of various genetic alterations and to develop tools (cellular and animal models) that are crucial for deciphering pathomechanisms, understanding the molecular defects and unveiling therapeutic targets.
We are still facing several challenges and bottlenecks: 1) a number of patients are still awaiting molecular diagnosis; 2) relevant biomarkers are scarce; 3) functions of the involved proteins and underlying pathomechanisms are still poorly understood … We previously have and continue to tackle several transverse processes (e.g. contractile dysfunction, defective mechanosensing, fibrosis …) using our specific expertise (nuclear envelop, nucleoplasm, extracellular matrix…).
Current research axes:
- Definition of genetic and clinical spectrum and delineation of natural history of these NMDs,
- Development of new tools to validate genetic variants identified through NGS (next generation sequencing),
- Deciphering pathomechanisms that affect skeletal and/or cardiac muscle, with the overall goal of identifying and assessing therapeutic options for these disorders.
Our work is carried out on biological material derived from patients (DNA, RNA cultured cells, or muscle biopsies), and on animal models developed in the team (mouse, zebrafish).
Noyau-MEC ©Astrid Brull
| Name | Position | ORCID |
|---|
- R. Ben Yaou, I. Dabaj, P. Yun, G. Norato, H. Xiong, et al.. First results from the international LMNA -related congenital and childhood onset muscular dystrophy retrospective natural history study. 22nd International Annual Congress of the World-Muscle-Society (WMS), Oct 2017, Saint Malo, France. 27, pp.S137-S138, 2017, ⟨10.1016/j.nmd.2017.06.165⟩. ⟨hal-03973439⟩
- Mathieu Cerino, Svetlana Gorokhova, P. Laforêt, R. Ben Yaou, Emmanuelle Salort-Campana, et al.. Genetic characterization of a French cohort of GNE -mutation negative inclusion body myopathy patients using exome sequencing. 22nd International Annual Congress of the World-Muscle-Society (WMS), Oct 2017, Saint Malo, France. 27, pp.S149, 2017, ⟨10.1016/j.nmd.2017.06.205⟩. ⟨hal-03973434⟩
- C. Macquart, M. Chatzifrangkeskou, M. Gotthardt, Gisèle Bonne, A. Muchir. Abnormal trafficking of connexin 43: A key element in the development of LMNA cardiomyopathy. 22nd International Annual Congress of the World-Muscle-Society (WMS), Oct 2017, Saint Malo, France. Neuromuscular Disorders, 27, pp.S139, 2017, ⟨10.1016/j.nmd.2017.06.171⟩. ⟨hal-03973377⟩
- I. Nelson, M. Jacquemont, A. Urtizberea, M. Renouil, A. Boland, et al.. A novel INPP5K mutation in a sibship from the Reunion Island. 22nd International Annual Congress of the World-Muscle-Society (WMS), Oct 2017, Saint Malo, France. Neuromuscular Disorders, 27, pp.S110-S111, 2017, ⟨10.1016/j.nmd.2017.06.071⟩. ⟨hal-03973393⟩
- I. Dabaj, R. Ben Yaou, C. Bönnemann, A. Nascimento, A. Rutkowski, et al.. Corticosteroid treatment in early-onset lamin A/C related muscular dystrophies. 22nd International Annual Congress of the World-Muscle-Society (WMS), Oct 2017, Saint Malo, France. Neuromuscular Disorders, 27, pp.S138, 2017, ⟨10.1016/j.nmd.2017.06.167⟩. ⟨hal-03973411⟩
- J. Bohm, R. Schneider, E. Malfatti, V. Schartner, X. Lornage, et al.. Integrated analysis of the large-scale sequencing project ``Myocapture'' to identify novel genes for myopathies. 22nd International Congress of the World Muscle Society, Oct 2017, Saint Malo, France. pp.S195, ⟨10.1016/j.nmd.2017.06.367⟩. ⟨hal-01741738⟩
- M. Kammoun, V. Veksler, J. Piquereau, Gisèle Bonne, M. Beuvin, et al.. TIEG1 is a novel regulator of muscle mitochondrial biogenesis. 22nd International Annual Congress of the World-Muscle-Society (WMS), Oct 2017, Saint Malo, France. Neuromuscular Disorders, 27, pp.S117, 2017, ⟨10.1016/j.nmd.2017.06.093⟩. ⟨hal-03973380⟩
- M. Saunier, C. Gartioux, M. Beuvin, N. Mougenot, G. Bonne, et al.. Collagen VI deficiency: The heart of the matter. 22nd International Annual Congress of the World-Muscle-Society (WMS), Oct 2017, Saint-Malo, France. Neuromuscular Disorders, 27, pp.S106, 2017, ⟨10.1016/j.nmd.2017.06.057⟩. ⟨hal-03996987⟩
- M Garibaldi, J Rendu, E Lacene, G Brochier, Maud Bauvin, et al.. Morphological spectrum of RYR1 recessive myopathies: clinical and genetic correlation. Congress of the World Muscle Society, Oct 2017, Saint Malo, France. 2017. ⟨hal-03968355⟩
- V. Mariot, R. Joubert, C. Hourdé, L. Féasson, M. Hanna, et al.. Myostatin expression levels in neuromuscular diseases participates in anti-myostatin clinical failure. Neuromuscular Disorders, 2017, 27, pp.S231. ⟨10.1016/J.NMD.2017.06.490⟩. ⟨hal-03158311⟩
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Our last work on the OJRD
A guide to writing systematic reviews of rare disease treatments to generate FAIRcompliant datasets: building a Treatabolome