In response to injury, Muscle Stem Cells (MuSCs) exit quiescence, proliferate and differentiate to form new myofibers, while also repopulating the stem cell niche in preparation for future injuries. The transitioning between cell fates that allow MuSCs to regenerate the muscle fibers involves temporal ordered changes in gene expression that occur in response to environmental cues. The environmental cues are interpreted by the epigenetic machinery in the cell to establish transcriptional permissive or transcriptional repressive states at individual genes. The Dilworth lab is working to understand the role for different epigenetic enzymes in regulating the transitions between MuSC cell fates. In this seminar, characterization of the role for the H3K27me3 demethylase JMJD3 and the transcription elongation factor NELF in regulating MuSC-mediated regeneration will be presented.
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